Table 2.

Analysis of passive and active parameters of induced action potentials from sham-transfected (left) and forced Kv7.2/7.3 co-expressing (right) CTR33Qn1-derived neurons during 1 (upper), 2 (middle) and 3 (lower) weeks of neuronal differentiation post-plate down in vitro

Week		Action potential parameters	Sham transfected			+ Kv7.2/7.3
			Mean	SEM	n	Mean	SEM	n
1	Passive	Vm (mV)	− 30.2	1.8	17	− 30.9	2.0	20
		Rin (GΩ)	1.65	0.14	16	1.12	0.11	17
		Cp (pF)	14.1	2.0	13	13.7	1.4	15
	Spike analysis	Threshold (mV)	− 30.6	2.4	16	− 27.9	1.7	16
		Overshoot (mV)	29.0	4.5	16	25.2	4.0	16
		Afterhyperpolarization (mV)	− 57.0	4.3	16	− 54.3	2.7	16
		Amplitude (mV)	86.0	6.4	16	79.5	5.2	16
		Depolarization rate (V/s)	70.0	12.2	16	62.3	9.9	16
		Repolarization rate (V/s)	− 49.6	9.5	16	− 38.6	6.7	16
		Half-width (ms)	2.9	0.4	16	3.1	0.3	16
2	Passive	Vm (mV)	− 31.4	2.3	14	− 37.8	2.6	16
		Rin (GΩ)	1.36	0.19	12	0.88	0.12	11
		Cp (pF)	10.9	2.8	8	17.5	4.2	9
	Spike analysis	Threshold (mV)	− 38.0	3.0	12	− 30.9	3.2	11
		Overshoot (mV)	33.2	6.9	12	37.4	6.9	11
		Afterhyperpolarization (mV)	− 64.3	5.7	12	− 56.1	2.4	11
		Amplitude (mV)	97.5	11.6	12	93.5	6.7	11
		Depolarization rate (V/s)	83.0	14.0	12	94.2	18.4	11
		Repolarization rate (V/s)	− 56.2	12.6	12	− 45.8	8.8	11
		Half-width (ms)	3.0	0.5	12	2.8	0.3	11
3	Passive	Vm (mV)	− 38.5**	1.7	16	− 45.5**	1.7	11
		Rin (GΩ)	0.79	0.06	12	0.61	0.05	10
		Cp (pF)	17.1	2.8	11	16.3	2.5	10
	Spike analysis	Threshold (mV)	− 33.0	2.5	12	− 32.4	3.9	10
		Overshoot (mV)	34.19	5.1	12	42.2	3.8	10
		Afterhyperpolarization (mV)	− 56.0	5.2	12	− 60.7	2.9	10
		Amplitude (mV)	90.2	7.8	12	102.9	3.3	10
		Depolarization rate (V/s)	84.8	10.5	12	104.7	10.0	10
		Repolarization rate (V/s)	− 40.9	5.0	12	− 49.4	6.4	10
		Half-width (ms)	3.0	0.6	12	2.9	0.6	10

Vm membrane potential, Rin input resistance, Cp whole-cell capacitance

**Significantly different from each other at p < 0.01