FIG 4.
Eye titers of challenge virus shed from the corneal epithelium. Groups of 10 BALB/c mice immunized with the high (A), medium (B), or low (C) dose of virus vaccine or control supernatant as described in the legend to Fig. 3 were challenged with HSV-1 strain mP (4 × 105 PFU/eye) 4 weeks postimmunization. The eyes of 6 mice per group were swabbed at the indicated times, and the titers of challenge virus in them were determined. The experiment was repeated once. The eye swab data represent the geometric means ± SEM of the combined results from the two independent experiments (total number of mice, 12 per group). The control group was the same for all three graphs. *, P = 0.0221; **, P = 0.0044; and ***, P = 0.0002 to 0.0001 for KOS-NA compared with the ICP0− virus. P = 0.0013 on day 3 at the high dose and P = 0.0019 on day 4 at the medium dose for KOS-NA compared with the ICP8− virus. The dashed lines indicate limits of detection.