FIG 5.

Expression of A3G and REDD1 in primary human PBL and comparison to that in Vero cells. (A) The expression of A3G and REDD1 in unstimulated PBL and in PBL stimulated with PHA or IL-2 48 h was determined by Western blotting. Western blots from three independent experiments (with PBL from three independent donors) were quantified, and mean values relative to GAPDH normalized to those for unstimulated PBL are presented. (B) Comparison of A3G expression in primary human PBL (lane 1, unstimulated; lane 2, IL-2 stimulated), Vero-023 cells (lane 3), and Vero-A3G cells (lane 4).