FIG 1.

Experimental workflow and sample preparation. (A) Detailed scheme of the interaction proteomics workflow. The KSHV lytic cycle was induced by using RTA and SB in latently infected HuARLT2 cells or uninfected control parental cells. After 48 h, endogenous K15 protein was precipitated from both induced and uninduced samples by using a rat anti-K15 mAb (clone 6E7). (B) Western blot (WB) analysis, using antibody 10A6 to pK15, of a small aliquot of the IP preparation used for MS/MS analysis. Experiments were performed in two independent biological replicates.