FIG 3.

UL148A downregulates full-length MICA alleles but not the truncated allele MICA*008. The indicated HFFs were mock infected, BAC2 infected, or infected with one of the following BAC2 deletion mutants: BAC2 ΔUL148A or BAC2 ΔUL148B. Cells were harvested, and MICA surface expression was assessed by flow cytometry. (A to C) FLS3 HFFs overexpressing MICA*004-HA, harvested 48 hpi. (D to F) FLS3 HFFs overexpressing MICA*008-HA, harvested 48 hpi. (G to I) FLS1 HFFs (MICA*004/*009:01-*049), harvested 24 hpi. Black histograms represent MICA staining of mock-infected cells, red histograms represent MICA staining of cells infected with the indicated virus, and gray-filled histograms represent isotype control staining of mock-infected cells, which produced similar results for all cells.