FIG 4.

nsP2 inhibits JAK/STAT inhibition by inducing the nuclear export of pSTAT1. Blocking nuclear export indicates the functional nuclear import of STAT1. Vero cells were transfected with either mCherry-2A-nsP1, mCherry-2A-nsP2, or one of its mutants; 16 hpt, the cells were mock treated or treated with the nuclear exportin CRM1 blocker LMB for 3 h prior to 30-min stimulation with IFN-β. The cells were fixed, permeabilized, and stained for pSTAT1. The bars represent the means from 3 independent experiments, and the error bars indicate SEM. The asterisks indicate significant differences as a result of LMB treatment (Student's t test; P < 0.05).