FIG 4.
MxB reduces viral gene expression and amplification of the herpesvirus genome. (a) HSV-1 capsid protein (anti-VP5) expression in A549 cells was analyzed by Western blotting at 24 hpi (MOI of 0.1). Shown is a blot representative of two independent experiments. (b) Expression of immediate early genes of HSV-1 (ICP0) and MCMV (ie2) in infected U87MG or A549 cells was measured by RT-qPCR. CT values were normalized to actin and plotted relative to the ΔCT values determined for the infected control cell line. Error bars represent geometric means of results from three independent experiments with technical duplicates. (c and d) Viral genome equivalents relative to GAPDH as a host-specific probe of HSV-1-infected (c) or MCMV-infected (d) A549 cells (MOI of 1). Error bars represent the SEM of (c) three independent HSV-1 experiments with technical quadruplicates or (d) two independent MCMV experiments with technical duplicates. Statistical analysis was performed using an unpaired t test for each time point. Significance is depicted relative to the control cells. ***, <0.001; **, <0.01; *, <0.1; ns, nonsignificant.