FIG 3.

HCoV OC43 spike and nucleocapsid proteins are associated with axons in vivo. Brains were collected from PFA-perfused mice 5 days after intracerebral inoculation, sagitally sectioned, and stained for either βIII-tubulin and nucleocapsid protein N (A) or spike glycoprotein and nucleocapsid (B) and then analyzed by confocal microscopy. Note that in panel B the image is a representative image taken in the hippocampal region of the brain. The insets on the right represent zoomed images of the areas delimited by the blue dotted boxes.