FIG 3.

Mutation of TAATGARAT motif has no effect on DEX-mediated activation of IEtu1 promoter. (A) Schematic of IEtu1 promoter and IEtu1 Collapsed construct. The IEtu1 TAATGARAT sequence (10) and ΔTAATGARAT mutant used for this study are shown. (B) Neuro-2A cells were transfected with IEtu1 Collapsed or ΔTAATGARAT luciferase constructs (0.5 μg DNA) or cotransfected with GR expression construct (1.0 μg) using Lipofectamine 3000. Where indicated, DEX was added at 16 h after transfection. Dual-Luciferase activity was performed as previously described at 40 h after transfection (34). The results are averages ± SD from 3 independent experiments. *, P < 0.05 by Student t test.