FIG 7.

½ GREs modulate DEX-mediated activation of 3′-DRR sequences. (A) Luciferase constructs used to examine the effects of GREs and ½ GREs on DEX-mediated activation. (B) Neuro-2A cells were transfected with the designated luciferase construct (0.25 μg DNA) using Lipofectamine 3000 and were designated the GR expression construct (1.0 μg), as described in Materials and Methods. Empty vector was included to maintain the same DNA concentrations for each sample. Where indicated, DEX was added at 16 h after transfection. Dual-Luciferase activity was performed at 40 h after transfection as previously described (34). The results are averages ± SD from 3 independent experiments, and an asterisk denotes significant differences between other samples (P < 0.05 by Student t test).