FIG 1.

Effect of MxB depletion on IFN-α2b inhibition of HIV-1/VSV G infection in HT1080 cells. (A) Illustration of the gRNA target sites (in blue) in the MxB gene. The first 5 exons (I to V) of MxB are shown. The boundary of adjacent exons is specified by the nucleotide positions in MxB mRNA (GenBank accession number NM_002463.1). The ATG start codon (at nucleotide position 105) is highlighted in red. (B) Levels of MxB and STAT1 in control cells (Con) and cells stably transduced with Cas9/gRNA in the presence and absence of IFN-α2b (500 U/ml). The levels of tubulin were also determined as the internal control. (C) Inhibition of HIV-1/VSV G infection by IFN-α2b (500 U/ml and 1,000 U/ml). The levels of infectious HIV-1 were determined by infecting TZM-bl indicator cells. Suppression of HIV-1 infection in the control and knockout cell lines was calculated by dividing the levels of HIV-1 production with IFN-α2b treatment by those without IFN-α2b treatment. The data shown are the averages from three independent infections of HT1080 cells. Error bars indicate standard deviations. ns, not significant.