FIG 3.

Knockout of MxB alleviates inhibition of HIV-1 reporter virus by IFN-α2b. IFN-α2b (500 U/ml) was used to treat SupT1 cells, including control and MxB/gRNA1, MxB/gRNA2, or STAT1/gRNA knockout SupT1 cells. Sixteen hours later, cells were infected with HIV-1 reporter viruses NLENY1 or NLENY1-ES/VSV G. The number of infected (YFP-positive) cells was scored with flow cytometry. (A) The results of one representative infection experiment are shown. (B) The averages from three independent experiments are summarized in the bar graph. The values of IFN-α2b inhibition were calculated as described in the legend to Fig. 1C. Error bars indicate standard deviations. *, P < 0.05; **, P < 0.005. SSC-A, side scatter area.