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Antigenic characterization of gB detected in the oligomeric change assay. HSV-1 KOS was treated at pH 5.0 or 7.4 for 10 min and then neutralized to pH 7.4 for 10 min if testing reversibility. Virions were then treated with 1% SDS and analyzed under “native” SDS-PAGE analysis, followed by Western blotting with the antibody indicated above each panel. **, Oligomeric gB; *, monomeric gB; +, fragmented gB. MW markers in kilodaltons are shown at the left.
