FIG 4.

Effect of elevated temperature on acid-induced conformational changes of HSV-1 gB. HSV-1 virions were treated at pH 7.4 or 5.0 at the indicated temperature for 10 min. To test reversibility, pH 5.0-treated samples were neutralized back to pH 7.4 for 10 min at 37°C. Samples were blotted immediately to nitrocellulose membrane and probed at neutral pH with gB-specific MAb H126 or SS10. (b) 1% SDS was added, and samples were resolved under “native” PAGE conditions, followed by Western blotting with MAb H1359 to gB. MW markers in kilodaltons are shown on the left.