AeAV is infectious to Aedes cell lines but does not replicate in Huh-7, Vero, and BSR vertebrate cell lines. (A) RT-qPCR of AeAV genome and anti-genome in a 5-day time course in A. aegypti Aa20 cells and A. albopictus C6/36 cells. Error bars represent the standard errors of the means (SEM) from three biological replicates. (B) RT-PCR of AeAV genome in a 7-day time course in human hepatocellular carcinoma cells (Huh-7), African green monkey cells (Vero), and baby hamster kidney (BSR). M, mock-infected cells.