FIG 1.

Design of a biased genetic screen for the identification of novel C. elegans genes involved in antiviral RNAi. (A) Schematic structure of plasmid constructs to be used to create the reporter transgene array for biased genetic screen. HIP, heat-inducible promoter; protein A, the replicase of Flock house virus; Rz, self-cleaving ribozyme sequence derived from hepatitis D virus, which functions to remove all nonviral sequence at the 3′ end of the FR1gfp primary transcripts; GFP, the coding sequence of enhanced green fluorescence protein; Psur-5, the promoter of the endogenous gene sur-5; UTR, the 3′-end untranslated region of unc-54; Pmyo-2, the myo-2 promoter which directs gene expression in pharyngeal muscles. (B) The workflow for identification of worm genes required for antiviral RNAi.