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. 2018 Aug 16;92(17):e00416-18. doi: 10.1128/JVI.00416-18

FIG 5.

FIG 5

Identification of rrf-1 and rde-3 alleles that are defective in antiviral RNAi. (A) Identification of point mutations in rrf-1 coding sequence that disrupt rrf-1 function in antiviral RNAi. Shown here is the rrf-1 cDNA sequencing results for rrf-1 alleles 1025a and 1026e. The rrf-1 coding sequence in wild-type worm N2 was sequenced as a reference. (B) Point mutations in rde-3 allele 1031b that disrupt antiviral RNAi. The rde-3 coding sequence in wild-type worm N2 was sequenced as a reference. (C) Ectopic expression of wild-type rde-3 restored antiviral RNAi in worm mutants containing the 1031b allele. (Upper) Structure of plasmid construct that expresses wild-type rde-3. (Lower) Visualization of antiviral RNAi in worms containing the 1031b allele. Worms marked with red fluorescence in the head region contain a transgene that expresses wild-type rde-3.