TABLE 1.
Loss of antiviral RNAi alleles isolated in the biased genetic screen
| Candidate gene | Allele | Strength of green fluorescencea | Temp sensitivityb | Developmental defect |
|---|---|---|---|---|
| asd-1 | 1026f | ++++ | + | Reduced brood size |
| 1027e | +++ | + | Reduced brood size | |
| 1103a | ++++ | + | Reduced brood size | |
| 1026g | ++++ | + | Reduced brood size | |
| asd-2 | 1026b | ++++ | − | − |
| 1028g | ++++ | − | − | |
| 1029e | ++++ | − | − | |
| asd-3 | 1029c | ++++ | − | − |
| 1031e | ++++ | − | − | |
| 1102b | +++ | − | − | |
| asd-4 | 1025b | +++ | − | − |
| 1028f | ++++ | − | − | |
| 1029b | ++++ | − | − | |
| asd-5 | 1026a | ++++ | − | Reduced brood size |
| 1031a | ++++ | − | Reduced brood size | |
| 1031f | ++++ | − | Reduced brood size | |
| asd-6 (rde-3) | 1031b | ++++ | − | − |
| asd-7 | 1028j | ++++ | − | − |
| asd-8 | 1030d | +++ | − | − |
| asd-9 | 1026d | +++ | − | − |
| asd-10 | 1105a | +++ | − | Reduced brood size |
| asd-11 | 1030c | +++ | − | High frequency of males |
| asd-12 | 1026c | +++ | − | − |
| asd-13 (rrf-1) | 1025a | +++ | − | − |
| 1026e | +++ | − | − |
a
The GFP expression level in drh-3 and rrf-1 mutants was used as a reference. Candidate mutants that produce green fluorescence at a level higher than or equal to that in rrf-1 mutants carrying the same ty48 transgene array are marked as +++. Candidate mutants producing green fluorescence at a level higher than or equal to that in drh-3 mutants are marked as ++++.
b
Candidate mutants that are sterile at 25°C are defined as temperature-sensitive mutants.