FIG 5.

Humoral immune responses in ferrets induced by the different VSV*ΔG replicons and viral load after infection. (A) Schematic overview of the immunization-and-infection strategy and blood sampling time points. Naive ferrets were immunized twice with 10⁸ FFU VSV*ΔG replicons intramuscularly. All animals were challenged intranasally with 10⁵ TCID₅₀ A/Mexico/InDRE4487/2009 and sacrificed for virus titration on day 3 after infection. (B and C) Kinetics of total antibody titers (B) and NI titers (C) against pdmH1N1 in animals immunized with matched or mismatched antigens. Log₂-transformed groups were compared using two-way ANOVA with a Tukey posttest. (D and E) Temperature changes (E) and clinical scores (F) measured over 3 days after infection. Symbols represent the means for each group (n = 4; postinfection N1 H5N1, n = 2), and error bars indicate the standard deviations of the means. (F and G) Virus titers in the nasal turbinates (F) and lungs (G) in terms of TCID₅₀ per gram on MDCK cells. Symbols indicate data for individual animals, and black bars represent the respective means.