TABLE 3.
Substitution of the E gene changes the replication capacity of Tembusu viruses in ducks
| Virus | Infection route | Test timea | Virus titer (log10 TCID50/0.1 g)b |
|||
|---|---|---|---|---|---|---|
| Spleen | Lung | Kidney | Ovary | |||
| FX2010 | i.m. | 3 dpi | 2.89 ± 0.78c | 1.33 ± 0.87 | 2.17 ± 1.25 | 3.27 ± 0.27 |
| FX/MME | i.m. | 3 dpi | 4.28 ± 0.53 | –/–/– | –/0.67/2.00 | 2.78 ± 0.19 |
| MM1775 | i.m. | 3 dpi | 3.72 ± 0.75 | –/–/– | –/–/– | –/–/0.67 |
| MM/FXE | i.m. | 3 dpi | 3.17 ± 0.76 | 2.67/2.33/– | –/2.33/– | 2.50 ± 0.33 |
| FX2010 | Contact | 6 dpc | 2.72 ± 0.25 | 2.33 ± 0.87 | 2.00 ± 0.44 | 3.00 ± 0.19 |
| FX/MME | Contact | 6 dpc | –/–/– | –/–/– | –/–/– | –/–/– |
| MM1775 | Contact | 6 dpc | –/–/– | –/–/– | –/–/– | –/–/– |
| MM/FXE | Contact | 6 dpc | 4.83 ± 0.44 | 2.17 ± 0.58 | –/0.50/– | 2.57 ± 1.39 |
a
dpi, days postinoculation; dpc, days postcontact.
b
Where virus was detected in all three ducks sampled, the mean TCID50 ± the standard deviation is shown; otherwise, individual titers are shown for each animal. –, No virus was detected.