FIG 2.

Recombinant TEV sites provide selective cleavage of pUL36. (A) Experimental workflow of extracellular virus particle harvesting and processing. (B) On-particle cleavage of GFP-pUL36 using viruses encoding a TEV site at position A, B, or C (Fig. 1A) was monitored by Western blot analysis using an anti-pUL36 antibody that recognizes an N-terminal epitope and an anti-VP5 antibody as a loading control.