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pUS3 and pUL37 tegument proteins are tethered to capsids by pUL36. (A) Extracted dual-fluorescent extracellular virus particles encoding a TEV site at position C in pUL36 were incubated in the absence or presence of TEV protease, and representative images of fluorescence emissions are shown. (B) Fluorescent intensities from single particles are presented normalized to the cognate “− TEV protease” control (n ≥ 3). Error bars indicate standard errors of the means (****, P < 0.001).
