Table 1.
Metabolite levels in wild-type (WT) and engineered yeast strains in g/g CDW quantified by GC-MS.
| Squalene | 2,3-Oxidosqualene | Lanosterol | Ergosterol | Lupeol | |
|---|---|---|---|---|---|
| WT | 0.0248 (± 0.0078) |
n.d. | 0.01162 (± 0.00567) |
0.0473 (± 0.01844) |
n.d. |
| Vector control | 0.0168 (± 0.0020) |
n.d. | 0.009172 (± 0.00203) |
0.0332 (± 0.0061) |
n.d. |
| TkLUP | 0.0181 (± 0.0030) |
n.d. | 0.00803 (± 0.00148) |
0.0289 (± 0.0042) |
0.00016 (± 0.00008) |
| rox1::PGAL1-tHMGR PGAL10-ERG13 | 0.1539 (± 0.0126) |
n.d. | 0.03614 (± 0.01597) |
0.0404 (± 0.0038) |
n.d. |
| rox1::PGAL1-tHMGR PGAL10 ERG13 vector control | 0.1037 (± 0.0385) |
n.d. | 0.03089 (± 0.01019) |
0.0271 (± 0.0061) |
n.d. |
| rox1::PGAL1-tHMGR PGAL10-ERG13 TkLUP | 0.1486 (± 0.0439) |
n.d. | 0.02860 (± 0.00954) |
0.0417 (± 0.0092) |
0.00266 (± 0.00074) |
| rox1::PGAL1-tHMGR PGAL10-ERG13 PERG7Δ::PCTR3 0 μM CuSO4 |
0.1018 (± 0.0081) |
0.0418 (± 0.0191) |
0.10514 (± 0.03471) |
0.0416 (± 0.0057) |
n.d. |
| rox1::PGAL1-tHMGR PGAL10-ERG13 PERG7Δ::PCTR3 150 μM CuSO4 |
0.0442 (± 0.0018) |
0.1974 (± 0.0345) |
0.00645 (± 0.00290) |
0.01765 (± 0.0054) |
n.d. |
| rox1::PGAL1-tHMGR PGAL10-ERG13 PERG7Δ::PCTR3 375 μM CuSO4 |
0.0194 (± 0.0031) |
0.2996 (± 0.0867) |
0.00098 (± 0.00018) |
0.0027 (± 0.0003) |
n.d. |
| rox1::PGAL1-tHMGR PGAL10-ERG13 PERG7Δ::PCTR3 vector control 150 μM CuSO4 |
0.0152 (± 0.0009) |
0.0824 (± 0.0011) |
0.00330 (± 0.00012) |
0.0053 (± 0.0002) |
n.d. |
| rox1::PGAL1-tHMGR PGAL10-ERG13 PERG7Δ::PCTR3 TkLUP 150 μM CuSO4 |
0.057 (± 0.0097) |
0.1443 (± 0.0479) |
0.00463 (± 0.00040) |
0.0107 (± 0.0017) |
0.02032 (± 0.00472) |
g/g CDW (± standard deviation); standard deviation was calculated from n = 3 individual transformants using Student’s t test
n.d. not detectable, CDW cell dry weight