Fig. 1.

PCR products obtained using primers designed to be specific for each of the bovine granzyme genes and perforin (PFN). The sizes of the PCR products: granzyme A (A), 838 bp; granzyme O (O), 849 bp; granzyme B (B), 818 bp; granzyme H (H), 820 bp; granzyme K (K), 889 bp; granzyme M (M), 833 bp; PFN, 1275 bp. a A T. parva-specific CD8 T cell line (purity, 99%) harvested 7 days after antigenic stimulation was used as the source of template cDNA. Lanes on the left contain primers without template for the individual granzymes and those on the right include template cDNA. b Resting bovine peripheral blood mononuclear cells (PBMC) and purified populations (purity, > 98%) of activated T cell subsets (CD4, CD8 and γδ T cells) and activated NK cells harvested 7 days after antigenic stimulation were used as the source of template cDNA. As a control, cDNA isolated from Madin-Darby Bovine Kidney (MDBK) epithelial cells was included