Fig. 3.

Phylogenetic relationship of granzymes and their related genes from human, mouse and cattle. Phylogenetic analysis was performed on cDNA sequences and the tree was constructed with the neighbour-joining algorithm using Mega 7.0 software. Numbers represent percentage bootstrap values out of 2000 replications. The evolutionary distances were computed using the Maximum Composite Likelihood method and are in the units of the number of base substitutions per site. All positions containing gaps and missing data were eliminated. The three main phylogenetic groups are labelled as trypsin-like, chymotrypsin-like and metase. Bovine granzyme genes are highlighted in red boxes. Hs, Homo sapiens; Mm, Mus musculus; Bt, Bos Taurus; GZM, granzyme; CTSG, cathepsin G; CMA, mast cell a-chymase; DDN, duodenase; ZAU1, azurocidin 1; PRTN3, proteinase 3; ELANE, neutrophil elastase preproprotein; CFD, complement factor D