Fig. 4.

Amino acid sequence alignment of bovine, human, mouse and pig granzymes showing conservation of functionally relevant amino acid residues. Signal sequence cleavage sites are indicated by an asterisk; conserved consensus sequences at the N-terminus at positions 1–4 (IIGG) and 9–16 (PHSRPYMA) are shown in yellow; the three amino acids of the catalytic triad are shown in red; cysteine residues that form disulfide bonds are shown in blue. Substrate-determining residues in S1, S2 and S3 are shown in green. Numbering of residues is based on that used for the chymotrypsinogen amino acid sequence. Dot, identical; dash, gap. Hs, Homo sapiens; Mm, Mus musculus; Bt, Bos Taurus; Ss, Sus scrofa; GZM, granzyme