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. 2018 Jun 8;70(9):585–597. doi: 10.1007/s00251-018-1062-6

Table 1.

PCR primers designed for detection of bovine granzymes and perforin

Primers Sequences (5′–3′)
Granzyme A (For) ATTGATTGATGTGGGGACAC
Granzyme A (Rev) AAAAAGTAACAGCAAATGAAATACAA
Granzyme O (For) AGTCTCCATATGTGAATAACAGGAG
Granzyme O (Rev) CCCTTTCACTTGGTTACTTCG
Granzyme B (For) CATCCTGGGCAGTCTTTCTA
Granzyme B (Rev) CCTGCAGTGTGATTCTGGAT
Granzyme H (For) CTGACCTGGGCAAATCTTCT
Granzyme H (Rev) GGACAATGGTCAGTGCAGAG
Granzyme K (For) TTCCTTTGCCAATACAGTCAG
Granzyme K (Rev) AGCAGCTGATAGAGCCAAGA
Granzyme M (For) GAGGCCCCCCAGATCCAAG
Granzyme M (Rev) CCCCTTGGAACACAGAATCA
Perforin (For) CAGGGTGGTCAAGCTAGAGG
Perforin (Rev) AGGTGAGGCAAGCATTTGAC