Fig. 2.

CED-10 protects DAn from α-SYN-induced DA cell death in C elegans. Representative RNAi empty vector (EV) fed worms expressing GFP and α-SYN specifically in DAn (Pdat-1:: α-SYN + Pdat-1:: GFP) at L4 + 7days (9 days post hatching) and fed with empty vector (EV) or ced-10 RNAi clones. Filled white arrowhead labels healthy neurons whereas degenerated or missing neurons are labeled with an open arrow. a ced-10 depletion reduces the amount of DAn per worm and the expression of the CED-10::CFP transgene {baEx167 [Pced-10::CFP::ced-10]} delays the DA cell death at the same age. Magnification bar is 30 μm. b Percentage of Pdat-1:: α-SYN + Pdat-1:: GFP worms non-depleted and ced-10 depleted by RNAi, that had the full complement of six anterior DAn at day 7 post L4. The transgenic derivative strain UA281 expressing CFP::CED-10 (CED-10 wild type), carrying the array baEx167 [Pced-10::CFP::ced-10] ameliorates the DA cell death. Data are mean ± SEM. Statistics were obtained by comparing ced-10 RNAi depleted worms or worms containing the CFP::CED-10 array with the corresponding EV fed animals. Statistics: ***P < 0.001, one-way ANOVA, Tukey’s post hoc test. Number of animals is 30–35 per condition, and the experiment was repeated three times independently