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. 2018 Jun 6;5(8):1701079. doi: 10.1002/advs.201701079

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© 2018 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Optimization of formulations of CS‐g‐bPEI/pDNA NPs with respect to transfection efficiency and cytotoxicity. Effects of DS of bPEI on CS‐g‐bPEI: A) fluorescent images; B) transfection efficiency; and C) LDH cell cytotoxicity. Effects of CS‐g‐bPEI40%:pDNA weight ratio: D) fluorescent images; E) transfection efficiency; and F) LDH cell cytotoxicity. *: Statistically significant (P < 0.05); ‡: Statistically significant versus CS group (P < 0.05). £: Statistically significant versus bPEI group (P < 0.05).