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α- and β-Syntrophins bind specifically to different repeats. Peptides corresponding to the SBSs in SLRs were used to pull down syntrophins from mouse muscle homogenates. The associated syntrophins were identified by western blot. Hmg—muscle homogenate before pull down assay, Rnd 17—peptide from SLR-17 with amino acids in random order, SLR 15—negative control peptide with SLR 15 sequence that does not contain a SBS.
