Fig. 3.

Inhibitory effect of PL on LPS-induced neuronal cell damage and amyloidogenesis. Cresyl violet staining for the observation of neuronal cell damage in the hippocampal CA3 zone was performed on mice brain sections (a). Thioflavin S staining for accumulation of Aβ in the hippocampus was performed on mice brain sections (b). Immunostaining of Aβ protein in the hippocampus was performed on mice brain sections with specific primary antibody and the biotinylated secondary antibody (c). All stains were performed on 20-µm-thick sections of mouse brains (n = 3). The level of Aβ₁₋₄₂ in mouse brains was measured by ELISA (n = 3) (d). The level of β-secretase activity in mouse brains was measured by β-secretase activity kit (n = 5) (e). The level of γ-secretase activity in mouse brains was measured as described in the “Materials and Methods” section (n = 5) (f). Data are expressed as the mean ± S.E.M. (t test, *p < .05 vs. control, ^#p < .05 vs. LPS). The expressions of APP and BACE1 were detected by Western blotting using specific antibodies in mouse brains (g). Each blot is representative of three experiments