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Inhibitory effect of PL on LPS-induced glial cell activation and neuroinflammation. Immunostaining of iNOS, COX-2, GFAP, and IBA-1 proteins in the hippocampus was performed on 20-µm-thick sections of mouse brains with specific primary antibodies and the biotinylated secondary antibodies (a) (n = 3). The expressions of iNOS, COX-2, GFAP, and IBA-1 were detected by Western blotting using specific antibodies in mouse brains. Each blot is representative of three experiments (b)
