Fig. 5.

Inhibitory effect of PL on LPS-induced activity of NF-κB. To observe the activity of NF-κB, DNA-binding assays in the mouse brain (a), cultured astrocyte primary (b), and microglial BV-2 cells (c) was performed by EMSA as described in the “Materials and Methods” section. The expressions of NF-κB-related proteins, such as IκB, p-IκB, p50, and p65, were detected by Western blotting using specific antibodies in the mouse brain (d), cultured astrocyte primary (e), and microglial BV-2 cells (f). Each blot is representative of three experiments