Fig. 8.

Binding assay between PL and p50 of NF-κB subunit. 3D structure of PL (a). To find an interaction between the PL and p50 of NF-κB subunit, we performed a pull-down assay, BiaCore assay, and docking model assay. In the pull-down assay, PL was conjugated with CNBr-activated Sepharose 4B beads and p50 protein bound PL-Sepharose 4B beads (b). The docking model of PL with p50 was performed as described in the “Materials and Methods” section (c). Difference in the inhibitory effect of PL on LPS-induced NO generation between normal RAW 264.7 cells and p50 mutant plasmid transfected RAW 264.7 cells (d). Data are expressed as the mean ± S.E.M. (n = 4, t test, *p < .05 vs. normal control, ^#p < .05 vs. normal LPS, ⁺p < 0.05 vs. each normal group)