Fig. 4.

Effect of IL-1RA on HUVEC migration. a The influence of different concentrations of IL-1RA on HUVEC migration was assessed using the BD Bio-Coat Matrigel invasion assay system. D, 0 ng/mL IL-1RA; D1, 1 ng/mL IL-1RA, D2 10 ng/mL IL-1RA; D3, 100 ng/mL IL-1RA. b Effect of co-culture with gastric cancer cells (gastric cell lines MKN45 and NUGC-4) treated with no rIL-1RA (leftmost column) or different concentrations of rIL-1RA on HUVAC migration: columns with fine diagonal lines to the left 1 ng/mL rIL-1RA, columns with large diagonal lines to the left 10 ng/mL rIL-1RA, columns with fine diagonal lines to the right (rightmost column) 100 ng/mL rIL-1RA. W, Co-culture with MKN45; W1, with MKN45 + 1 ng/mL IL-1RA; W2, with MKN45 + 10 ng/mL IL-1RA; W3, with MKN45 + 100 ng/mL IL-1RA. C, Co-culture with NUGC-4; C1, with NUGC-4 + 1 ng/mL IL-1RA; C2, with NUGC-4 + 10 ng/mL IL-1RA; C3, with NUGC-4 + 100 ng/mL IL-1RA. Asterisks indicate significant difference from the control (0 ng/mL rIL-1RA) at *P < 0.01, **P < 0.05. Columns and whiskers Mean and SD