Figure 3.

Effects of IL-6 on immune cells tumor infiltration in CRC mouse models. (A) The tumor-infiltrating immune cells were measured by flow cytometry. Gating plots for T cells are shown. (B, C) The ratio of CD8⁺ T cells was significantly increased in the tumor tissues with IL-6 deficiency but was decreased in the tumors with IL-6 overexpression. (D, E) The number of CD4⁺ T cells in tumors with IL-6 deficiency was significantly enhanced but was decreased in tumors with IL-6 overexpression. ** P value less than 0.01; *** P value less than 0.001; **** P value less than 0.0001; WT – wild-type; EV – empty vector; OE – overexpression; sample size of every experimental group was 10 mice.