Fig. 4. miR-451's antagomir reverses ovariectomy (OVX)-induced bone loss and improves bone strength in mice. (A) Reduced miR-451 expression in bone following injection of its antagomir into six-week-old female Balb/c mice. (B) Representative micro-CT images of the femoral epiphyses in sham and OVX mice. (C–F) Measurement of the structural micro-CT parameters, including BV/TV, Tb.N, Tb.Sp, bone strength, and bone stiffness for (C–F) the femoral epiphyses, (G–I) tibial epiphyses, and (J–L) vertebrae. Femoral bone histomorphometric measures are also provided, including (M) the mineralizing surface (MS), (N) mineral apposition rate (MAR), and (O) bone formation rate (BFR). n = 15 mice/group. Data reported as means ± SEMs. *p < 0.05 versus matching PBS group, †p < 0.05 versus matching miR-C group.