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. 2018 Jul 10;9(8):1359–1368. doi: 10.1039/c8md00187a

Table 1. Primers used in qRT-PCR.

Gene Forward primer Reverse primer
Human BMP2 5′-ATG TTC GCC TGA AAC AGA GAC CCA-3′ 5′-CTT ACA GCT GGA CTT AAG GCG TTT C-3′
Human SMAD1 5′-CCG CCT GCT TAC CTG CCT CCT GAA-3′ 5′-GAA CGC TTC GCC CAC ACG GTT GT-3′
Human RUNX2 5′-TCT TCA CAA ATC CTC CCC-3′ 5′-TCT TCA CAA ATC CTC CCC-3′
Human ALP 5′-ACC TCG TTG ACA CCT GGA AG-3′ 5′-CCA CCA TCT CGG AGA GTG AC-3′
Human COL1A1 5′-GGG CAA GAC AGT GAT TGA ATA-3′ 5′-ACG TCG AAG CCG AAT TCC T-3′
Hsa-miR-451 5′-AAA CCG TTA CCA TTA CTG AGT T-3′ Universal adaptor PCR primer (GeneCopoeia)
Human GAPDH 5′-CGA AGT CAA CGG ATT TGG TCG TAT-3′ 5′-AGC CTT CTC GGT GAA GAC-3′
Murine Ywhaz 5′-TGC AAC GAT GTA CTG TCT CTT TTG-3′ 5′-CGG TAG TCA CCC TTC ATT TTC A-3′
Murine Runx2 5′-CCA GAA TGA TGG TGT TGA CG-3′ 5′-GGT TGC AAG ATC ATG ACT AGG G-3′
Murine Alp 5′-ATG GGC GTC TCC ACA GTA AC-3′ 5′-CTG AGT GGT GTT GCA TCG C-3′
Murine Col1a1 5′-TGG CAA AGA CGG ACT CAA C-3′ 5′-GGC AGG AAG CTG AAG TCA TAA-3′
Mmu-miR-451 5′-AAA CCG TTA CCA TTA CTG AGT T-3′ Universal adaptor PCR primer (GeneCopoeia)
Murine Gapdh 5′-GCC AAA AGG GTC ATC TC-3′ 5′-GTC TTC TGG GTG GCA GTG AT-3′