Figure 2.

Functional complementation of eif4e1 ^KO plants by eIF4E1 ^R allele. (a) Bolting of eif4e1 ^KO and complemented plants, 4 weeks after sowing. Columbia Wild‐type plants (Col WT) are used as control. Results are shown for three independent transgenic lines (1 to 3) expressing the eIF4E1^R construct in a eif4e1 ^KO background. Table legend shown under (b) applies also for (a). (b) Boxplot representation of the bolting time (in days after sowing) for the same genotypes as in (a). Results are averaged from 16 individual plants per genotype. (a) and (b) represent significantly different groups (P < 0.05). (c) In planta cap‐binding purification of eIF4E1 proteins. Total soluble protein extract from control and transgenic plants was purified on m7GTP‐agarose beads. After purification, the output fraction was analysed by Western blot using anti‐eIF4E1 antibody while equal loading control was checked on total protein (input) by Western blot for actin detection and by Ponceau staining for Rubisco protein detection.