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. 2018 Aug 10;9:1111. doi: 10.3389/fphys.2018.01111

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Copyright © 2018 Yamada, Himori, Tatebayashi, Yamada, Ashida, Imai, Akatsuka, Masuda, Kanzaki, Watanabe, Wada, Westerblad and Lanner.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ES treatment prevents the myosin loss in S-D muscles. Representative stain-free gel of myofibrillar proteins and immunoblots for fast type (f)-MyHC in skinned fibers in control (CNT) and steroid-denervation (S-D) rats with or without ES treatment (A). The ratio of MyHC to actin (B). Bars show the mean and SEM results from 14 to 18 fibers per group. ^∗P < 0.05 vs. CNT, ^#P < 0.05 vs. S-D. Representative stain-free gel of the total proteins in whole muscle homogenates (C). The expression levels of MyHC normalized to the total proteins (D). Bars show the mean and SEM results from 5 to 6 muscles per group. ^∗P < 0.05 vs. CNT, ^#P < 0.05 vs. S-D.