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. 2018 Aug 9;24:5533–5541. doi: 10.12659/MSM.909901

Figure 4.

Figure 4

Lipopolysaccharide (LPS) triggered epidermal growth factor receptor (EGFR) phosphorylation in hepatic stellate cells (HSCs) through the TLR4/c-Src signaling cascade. (A–C) Hepatic stellate cells (HSCs) were pretreated with AG1478 (2.5 μM and 5 μM) for 2 h. After LPS treatment for 15 min, the phosphorylation levels of EGFR (A), the expression of TLR4 (B), and phosphorylation level of c-Src (C) were detected using Western blot. (D, E) Small interfering RNA (siRNA) transfection did not reduce LPS (15 min)-induced increased expression of TLR4 and c-Src activation. (F) HSCs were pretreated with TAK-242 (a TLR4 inhibitor), or PP2 (a c-Src inhibitor) for 1 h, followed by LPS treatment for 15 min. Phosphorylation of EGFR was detected using Western blot. * P<0.05, ** P<0.01, *** P<0.001, vs. Ctrl group; ns – not significant vs. the LPS-treated group; ## P<0.01, vs. the LPS-treated group.