Table 1.

Cofilin-1 phospho-residues identified by mass spectrometry analysis from C2-WT, C2-H222P and C2-H222P cells treated with selumetinib

Serine	S3			S23			S41			S94			S156
Genotype	C2-WT	C2-H222P	C2-H222P (selumetinib)	C2-WT	C2-H222P	C2-H222P (selumetinib)	C2-WT	C2-H222P	C2-H222P (selumetinib)	C2-WT	C2-H222P	C2-H222P (selumetinib)	C2-WT	C2-H222P	C2-H222P (selumetinib)
A	×	×	×	×									×	×	×
B	×	×	×
C	×	×											×
D	×	×	×			×		×				×	×	×	×
Threonine	T25			T63			T88
Genotype	C2-WT	C2-H222P	C2-H222P (selumetinib)	C2-WT	C2-H222P	C2-H222P (selumetinib)	C2-WT	C2-H222P	C2-H222P (selumetinib)
A				×	×	×
B
C
D		x		×	×	×	×	×
Tyrosine	Y82			Y85			Y89			Y140
Genotype	C2-WT	C2-H222P	C2-H222P (selumetinib)	C2-WT	C2-H222P	C2-H222P (selumetinib)	C2-WT	C2-H222P	C2-H222P (selumetinib)	C2-WT	C2-H222P	C2-H222P (selumetinib)
A	×	×	×							×	×	×
B	×	×	×							×
C	×	×	×								×
D	×	×	×	×					×	×	×	×