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. 2018 May 22;27(17):2986–3001. doi: 10.1093/hmg/ddy190

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Figure 2. — Expression of PS loop rescues GSK3β mediated transport defects. (A–C) A representative larval segmental nerve from WT larva and larva expressing SGG^WT or SGG^ACTIVE stained with the synaptic vesicle marker CSP. Note that the WT nerve shows smooth staining. In contrast, larval segmental nerves expressing SGG^WT or SGG^ACTIVE show axonal blockages (arrows). Scale Bar = 10 µm. (D) Larval segmental nerves from larvae expressing hPSloop show smooth staining. (E and F) Larval segmental nerves expressing hPSloop and SGG^WT or SGG^ACTIVE also show smooth staining. (G) Larval segmental nerves from larvae expressing PSΔE9 show axonal blockages (arrows). (H and I) Larval segmental nerves from larvae expressing PSΔE9 and SGG^WT or SGG^ACTIVE show axonal blockages (arrows). (J) Quantification analysis shows that the extent of axonal blockages in SGG^WT (P = 0.02784), and SGG^ACTIVE (P = 0.00721) is significant, but the extent of axonal blockages in hPSloop is not (P = 0.09718). Note that while the extent of the decrease of axonal blockages in hPSloop; SGG^WT was not significant compared with SGG^WT (P = 0.2738), the extent of decrease of axonal blockages was significant in hPSloop; SGG^ACTIVE (P = 0.0130). N = 5 larvae for each genotype from at least three independent genetic crosses. Data are presented as mean ± SEM. (*P < 0.05, ns = non-significant, using two sided Student’s t-test). (K) Quantification analysis shows that the extent of axonal blockages in larvae expressing PSΔE9 is significant (P = 0.00134) compared with WT. The extent of axonal blockages in larvae expressing PSΔE9 and SGG^WT is significant compared with SGG^WT alone (P = 0.01847) but is not compared with PSΔE9 alone (P = 0.53979). N = 5 larvae for each genotype from at least three independent genetic crosses. Data are presented as mean ± SEM (*P < 0.05, **P < 0.005, ns = non-significant, using two sided Student’s t-test). (L) Quantification analysis shows that the extent of axonal blockages in larvae expressing PSΔE9 and SGG^ACTIVE is significantly increased compared with PSΔE9 alone (P = 0.03475) or SGG^ACTIVE alone (P = 0.000576). N = 5 larvae for each genotype from at least three independent genetic crosses. Data are presented as mean ± SEM (*P < 0.05, **P < 0.005, using Student’s t-test).