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. 2018 Aug 16;19:291. doi: 10.1186/s12891-018-2221-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Study design. The right knee joint had an intra-articular injection of MIA at day 0. The left knee had PBS as a control. Histological and immunohistochemical evaluations (hematoxylin and eosin/safranin O staining and calcitonin gene-related peptide staining) were performed at 5, 14, and 28 days post-injection. The pain avoidance behavior tests (incapacitance and von Frey) were performed as indicated