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. 2018 Jul 30;15(11):1241–1250. doi: 10.7150/ijms.25115

Figure 1.

Figure 1

Culture and identification of h-PDLSCs. (A) Primary cells derived from human periodontal ligament tissue (scale bar: 50 μm). (B) The immunophenotypes of h-PDLSCs were analyzed by flow cytometry using hMSC positive markers (CD44, CD73, CD90, and CD105) and hMSC negative markers (CD11b, CD19, CD34, CD45, and HLA-DR). (C) Alizarin Red staining after osteogenic induction for 4 weeks (scale bar: 50 μm). (D) Oil red O staining after adipogenic induction for 4 weeks (scale bar: 50 μm). (E) Alcian blue staining after chondrogenic induction for 4 weeks (scale bar: 20 μm).