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Overexpression efficiency and localization of YAP. (A) Levels of YAP mRNA were examined by qRT-PCR with GAPDH as a control. (***P<0.001). (B) Levels of YAP protein were examined by Western blotting with GAPDH as a control. (C) Localization of YAP was detected by immunofluorescence staining with blue DAPI nuclear counterstain (scale bar: 50 μm).
