Figure 3.

Overexpression of YAP prompted the proliferation of h-PDLSCs. (A) Cell proliferation was measured by EdU staining. The nucleus of EdU positive cells were red, and nucleus of all cells were stained with Hoechst blue. The number of stained cells was count under fluorescence microscope, and the percentages of proliferating cells were determined as EdU-positive cells/all cells. Data were means ± standard deviation (**P<0.01) (scale bar 100 μm). (B) Cell proliferation was measured by CCK-8. (*P<0.05, ***P<0.001) (C) Levels of P-Msk1, ERK, P-ERK1/2, P-MEK1/2, and P-P90RSK were examined by Western blotting with GAPDH as a control.