Table 1.
Primers and probes used for diagnostic and phylogenetic analysis
| Primer ID | Sequence | Annealing temp. | PCR | Reference |
|---|---|---|---|---|
| 16sU17F | AGAGTTTGATCCTGGCTCAG | 56 °C | First | [30] |
| 16sOR1198R | TTTCCTATAGTTCCCGGCATT | 56 °C | First & second | |
| 16sO79F | ATTAATGCTGAGCTTGCTTAGCAT | 56 °C | Second | |
| Otr47_263F | GTGCTAAGAAARGATGATACTTC | 54 °C | First | [31] |
| Otr1780R | AAATCGCCTTTAAACTAGATTTACTTATTA | 54 °C | First & second | |
| Otr47F | TAAAGGTTAAGTTTATGAAAAAGGCATTT | 54 °C | Second | |
| Otr56_498F | AATTAGTTTAGAATGGTTACCAC | 54 °C | First | |
| r56_585F | AATGTCTGCGTTGTCGTTGC | 54 °C | Second | |
| r56_2057 | TCCACATACACACCTTCAGC | 54 °C | First & second | [32] |
| OtsuFP630 | AACTGATTTTATTCAAACTAATGCTGCT | 60 °C | Real time | [5] |
| OtsuRP747 | TATGCCTGAGTAAGATACRTGAATRGAATT | 60 °C | Real time | [5] |
| OtsuPR665 | 6FAM-TGGGTAGCTTTGGTGGACCGATGTTTAATCT-TAMRA | 60 °C | Real time | [5] |