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. 2018 Aug 14;11(5):1232–1243. doi: 10.1016/j.tranon.2018.07.014

Figure 4.

Figure 4

Immunofluorescence was performed to examine the relationship between Calponin 3 and E-cadherin. (A) Knockdown of Calponin 3 in SNU-2414B cell line increased E-cadherin expression. (B) To obtain entirety of floating SNU-2404B cell colonies, Z-stacking was applied. Within a single spheroid population, Calponin 3 was mostly located in the outer shell of the spheroid, and E-cadherin remained in the inner shell of the aggregate. (C) The intensity of Calponin 3 (Red) and E-Cadherin (Green) signals was measured and graphed through one axis. (a, b) Two random points were selected on the mid-transverse section of SNU-2404B shCONT spheroid image (16 μm). Peaks on the graph indicated that Calponin 3 signal is located in the outer region compared to E-cadherin signal. (c, d) Two random points were selected on the bottom-transverse section of SNU-2404B shCONT spheroid image (32 μm). Peaks on the graph indicated that Calponin 3 signal is located outer region compared to E-cadherin signal.