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. 2018 Jul 13;14(10):1221–1231. doi: 10.7150/ijbs.25488

Figure 1.

Figure 1

MLN0128 and AZD1775 inhibit SCLC cell proliferation in vitro. A. Decreased SCLC cell proliferation upon AZD1775 or MLN0128 treatment. H69 and H82 cells were incubated with serial concentrations of MLN0128 or AZD1775 for 72 hours and cell viability was assessed by CCK-8 assay. Average viability of DMSO-treated samples was taken as 100%. Data points represent the mean ± SEM of triplicate experiments. B. Decreased colony formation in SCLC cells treated with AZD1775 or MLN0128 alone or in combination. H69 and H82 cells were plated in methylcellulose medium with DMSO, MLN0128 (50nM), or AZD1775 (1000nM for H69, 250nM for H82), or combination of both inhibitors and cultured for 14 days at 37°C; colonies were counted on Day 14. Left panels (C) show representative images at 40x magnification from different microscopic fields. Right bar graphs (D) indicate the number of colonies at each plate. Data are presented as the mean ± SEM of triplicate experiments. ****p < 0.001 compared with DMSO-treated cells. # p< 0.05, ## p< 0.01 single treatment with combination treatment.